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The Many Spheres in Which CO2 Chambers Show Their Strengths

The Many Spheres in Which CO2 Chambers Show Their Strengths
Without CO2 incubators, there would be no coronavirus vaccines today. They are also absolutely essential for cancer research. (Credit: BINDER)

Without CO2 incubators, there would be no coronavirus vaccines today. They are also absolutely essential for cancer research. Their multiple uses help save lives and cure many different diseases. We would like to introduce you to some of the interesting facets of CO2 incubators.

Sponsored by BINDER GmbH.

Regenerative Medicine

CO2 incubators are being used to conduct research in laboratories across the globe. The Bioscience Institute Middle East, which is among the world’s leading centers for regenerative medicine, is also using an incubator to process the body’s own cells as well as for plastic surgery applications. 

The cells–which are multiplied in an incubator–are also used in tissue repair as well as for orthopedic and dermatological treatments. The Bioscience Institute only uses skin and fat tissue specimens from adult (mature) cells. Using the body’s own–i.e., autologous–cells eliminates the risk of rejection while also preventing the complication of graft-versus-host disease (an unwanted reaction of the donor’s immune cells). 

To be even more specific: the CO2 incubators are predominantly used to incubate stem cells from mesenchyme tissue (undifferentiated connective tissue). 

Here is how it works: first, cells are extracted from fat tissue. This process is performed by means of enzymatic disaggregation (separation) using various steps of filtration and centrifugation. The crucial stage here is the expansion, i.e., extracting as many stem cells as possible, which is why it is absolutely essential to create the best possible growth conditions. 

Dr. Simona Alfano, a biologist at the Bioscience Institute, explained:

“When incubating the cells, it is vitally important for the selected parameters to remain exactly constant across all levels.”

And this is precisely where the CO2 chambers from BINDER come into their own–with their reproducible growth conditions, constant climatic conditions, low risk of contamination and high level of safety. 

Find out more about why the ph value is a key factor in cell and tissue cultures.

Utmost safety is essential while preparing stem cells. (Credit: Binder)
Utmost safety is essential while preparing stem cells. (Credit: BINDER)

The Coronavirus Pandemic

CO2 chambers also played an important role during the coronavirus pandemic: firstly, in the development of coronavirus vaccines and, secondly, to test drugs that may be used to treat COVID-19 on cells. 

For this work, the major pharmaceutical companies required huge volumes of cells–which they were able to acquire with the aid of an incubator. The newly developed active ingredients were then tested using the cells. 

The new vaccines used in the fight against the coronavirus were also repeatedly tested on cells in laboratories and evaluated. An incubator proved to be an essential piece of equipment in a laboratory–particularly during the coronavirus pandemic.  

Read more on premium equipment for virus research.

Space Biology

The Institute of Medical Engineering at the Lucerne University of Applied Sciences and Arts has been carrying out research in the field of space biology. The research team, led by Dr. Fabian Ille, is assisted in its work by a CO2 chamber. 

Cells from a bovine hoof are being incubated inside the cabinet at regular intervals until they are needed for a specific experiment. Recently, the cells were frozen and taken to the French city of Bordeaux by Dr. Simon Wüest and a team of researchers. 

The reason behind this trip was that the research team in Lucerne was selected by the European Space Agency (ESA) to take part in parabolic flights over the Atlantic.  Shortly before the parabolic flights, which lasted for a total of three hours, the cells were removed from the incubator and moved to flight hardware that had been prepared specifically for this purpose and was under controlled temperature conditions. 

The scientists from Lucerne wanted to use the parabolic flights to investigate how the cells respond and adapt to mechanical forces. These findings will help them in future attempts to cultivate cartilage that is of a stronger and better consistency, for example. In other words, it might be possible to remove cells from a patient, reproduce them with this innovative new method, and then use them again in the treatment of human patients. 

“Weightless conditions are helping us to make significant progress,” said Dr. Ille, reflecting on the research project so far.

In laboratory tests that have already been carried out, Wüest and Ille have been able to demonstrate in very broad terms that this process could work in the future. In these tests, weightless conditions were simulated using a random position machine. Here again, a CO2 chamber from BINDER was used.

Novespace. (Credit: Lucerne University of Applied Sciences and Arts)
Novespace. (Credit: Lucerne University of Applied Sciences and Arts)

The Requirements Placed on CO2 Incubators

Safety is the absolute top priority here. 180°C sterilization ensures, for example, that every trial series begins with a clean and fully sterile incubator. What’s more, the fanless design means that germs are not stirred up. 

The result is optimal cell growth and absolutely no contamination from airborne germs. A deep-drawn inner chamber without corners or edges also enables the incubator to be cleaned thoroughly with ease. It is therefore no surprise that major pharmaceutical manufacturers choose specifically to put their trust in CO2 incubators from BINDER.

BINDER CO2 incubators are the perfect combination of a range of solutions–180°C hot air sterilization, rapid control, fixture-free interiors and absolutely zero consumables. For optimal cell growth–safe, reliable, smart, economical–look no further than BINDER.